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primary polyclonal rabbit anti nlrp3  (Proteintech)


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    Structured Review

    Proteintech primary polyclonal rabbit anti nlrp3
    Primary Polyclonal Rabbit Anti Nlrp3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+nlrp3/NLRP3+Antibody/pm42867763-74-24-30
    Average 96 stars, based on 1680 article reviews
    primary polyclonal rabbit anti nlrp3 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Exploring the potential mechanisms of PFDoA-induced microglial pyroptosis using cell biology experiments, network toxicology and molecular docking
    Article Snippet: Perfluorododecanoic acid (PFDoA) is a perfluorocarboxylic acid containing 12 carbon atoms, which is widely found in food packaging materials, seafood, surface water and soil, posing a potential risk to food safety and human health.. PFDoA preferentially accumulates in the brain and could cause cognitive impairment, however, the underlying mechanisms remain unclear.. In this study, we examined the effects of PFDoA on microglial cytotoxicity, and elucidated the underlying mechanisms through a combination of in vitro experiments and bioinformatics analysis.

    Incubation:

    Article Title: Exploring the potential mechanisms of PFDoA-induced microglial pyroptosis using cell biology experiments, network toxicology and molecular docking
    Article Snippet: Perfluorododecanoic acid (PFDoA) is a perfluorocarboxylic acid containing 12 carbon atoms, which is widely found in food packaging materials, seafood, surface water and soil, posing a potential risk to food safety and human health.. PFDoA preferentially accumulates in the brain and could cause cognitive impairment, however, the underlying mechanisms remain unclear.. In this study, we examined the effects of PFDoA on microglial cytotoxicity, and elucidated the underlying mechanisms through a combination of in vitro experiments and bioinformatics analysis.

    Article Title: Dihydromyricetin improves DSS-induced colitis and behavioral disorders by regulating the microbiota-gut-brain axis balance and inhibiting the activation of NLRP3 inflammasome.
    Article Snippet: .. Colon tissues were blocked with 5% bovine serum albumin for 20 min, then incubated overnight at 4 ◦C with rabbit anti-NLRP3 and anti-ZO-1 primary antibodies (1:200; Proteintech). .. Brain tissues were similarly blocked and incubated with rabbit anti-NLRP3 and anti-Iba-1 primary antibodies (1:200; Proteintech).

    Article Title: The <scp>CCL11</scp> ‐ <scp>CCR3</scp> Axis Regulates the Aggravation of Neuroinflammation in Astrocytic Necroptosis After Cerebral Ischemia
    Article Snippet: Proteins were separated on 10% SDS- PAGE gels and transferred to PVDF membranes (PR05509, Milipore, America). .. Membranes were blocked with 5% skim milk (1 h, RT) and incubated overnight at 4°C with primary antibodies: rabbit anti- NLRP3 (1:500, 15101, Proteintech, China), rabbit anti- ASC (1:200, DF6304, Affinity, America), mouse anti- β- actin (1:1000, TA- 09, ZSBIO, China), rabbit anti- MLKL (1:200, 82090- 2- RR, Cell Signaling Technology, China), rabbit anti- RIPK1 (1:200, 28252- 1- AP, Proteintech, China), rabbit anti- RIPK3 (1:1000, nloaded from https://faseb.onlinelibrary.w iley.com /doi/10.1096/fj.202502723R R by R eadcube (L abtiva Inc.), W iley O nline L ibrary on [30/12/2025]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense 4 of 16 The FASEB Journal, 2026 Immunoway, China), rabbit anti- p- MLKL (1:500, Cell Signaling Technology), rabbit anti- p- RIPK1 (1:1000, Proteintech), rabbit anti- p- RIPK3 (1:1000, Immunoway), rabbit anti- CCL11 (1:500, Abcam, UK), rabbit anti- CCR3 (1:1000, Affinity, USA), and mouse anti- IL- 1β (1:500, Cell Signaling Technology).

    Article Title: Anesthetic gas sevoflurane postconditioning alleviates cognitive impairment induced by hemorrhagic shock and resuscitation
    Article Snippet: The proteins were separated by 10% or 12.5% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred to polyvinylidene fluoride membranes (Merck, Darmstadt, Germany). .. The membranes were blocked with 5% skim milk for 2 hours and incubated with specific primary antibodies, including mouse anti-SIRT1 (1:1000, CST, Boston, MA, USA, Cat# 8469, RRID:AB_10999470), rabbit antiHIF-1α (1:1000, absin, Shanghai, China, Cat# 120168, RRID:AB_3676379), rabbit anti-NLRP3 (1:1000, Affinity, Changzhou, China, Cat# DF7438, RRID:AB_2839376), rabbit anti-gasdermin D (GSDMD; 1:1000, Affinity, Cat# AF4012, RRID:AB_2846776), rabbit anti-Caspase-1 (1:1000, Affinity, Cat# AF5418, RRID:AB_2837902), mouse anti-IL-1β (1:1000, Affinity, Cat# BF8021, RRID:AB_3713524), and mouse anti-GAPDH (1:10,000, Proteintech, Wuhan, China, Cat# 60004-1-Ig, RRID:AB_2107436), overnight at 4°C. .. After incubation with the corresponding secondary antibodies: goat anti-rabbit IgG (H + L) (1:10,000, Thermo Fisher Scientific, Cat# 31460, RRID:AB_228341) and goat anti-mouse IgG (H + L) (1:10,000, Thermo Fisher Scientific, Cat# 31430, RRID:AB_228307) at 25°C for 1 hour with gentle shaking, the immunoreactive bands were visualized via chemiluminescence in Tanon full-automatic imaging system (Tanon, Shanghai, China).

    Article Title: Dihydromyricetin improves DSS-induced colitis and behavioral disorders by regulating the microbiota-gut-brain axis balance and inhibiting the activation of NLRP3 inflammasome.
    Article Snippet: Colon tissues were blocked with 5% bovine serum albumin for 20 min, then incubated overnight at 4 ◦C with rabbit anti-NLRP3 and anti-ZO-1 primary antibodies (1:200; Proteintech). .. Brain tissues were similarly blocked and incubated with rabbit anti-NLRP3 and anti-Iba-1 primary antibodies (1:200; Proteintech). .. After PBS washing, sections were treated with Cy3-labeled goat anti- rabbit secondary antibody (1:200; Proteintech) for 1 h at room temperature in the dark.



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